ATG7 anticorps (C-Term)

N° du produit ABIN349977

Cet anticorps anti-ATG7 est un anticorps Mouton Polyclonal détectant ATG7 dans WB et IHC. Adapté pour Humain.

Aperçu rapide pour ATG7 anticorps (C-Term) (ABIN349977)

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivité: Humain

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG7 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-ATG7 anticorps

Épitope: C-Term

Fonction: Sheep antibody to ATG7

Specificité: Specific for ATG7.

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): Other species not yet tested.

Purification: IgG

Immunogène: A synthetic peptide from c-terminal region of human ATG7 conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in rat and mouse.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur ATG7

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Autre désignation: ATG7

Sujet: This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2 GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex composed of ATG3 and ATG7 plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Widely expressed especially in kidney liver lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy